| Paper Abstract and Keywords |
| Presentation |
2006-01-27 10:25
Application of the synaptic bouton preparation to rat retinal ganglion cells
Tamami Motomura, Yuki Hayashida (Kumamoto Univ.), Norio Akaike (Kumamoto Health Science Univ.), Nobuki Murayama (Kumamoto Univ.) |
| Abstract |
(in Japanese) |
(See Japanese page) |
| (in English) |
The vibrodissociation method has been applied to various CNS regions, yet never applied to vertebrate retinas. This study is aimed to examine whether this method is valid to isolate ganglion cells from a mammalian retina. In perforated-patch whole-cell recordings, the isolated ganglion cells showed sustained as well as transient spiking in response to depolarizing constant current injections, and showed voltage-gated Na+ current in response to depolarization steps. Under current-clamp, the ganglion cells somata showed spontaneous IPSP-like events of which reversal potential was approximately -80 mV. The present study suggested that the vibrodissociation method is useful to isolate rat retinal ganglion cells attached with inhibitory synaptic boutons. |
| Keyword |
(in Japanese) |
(See Japanese page) |
| (in English) |
retina / ganglion cell / synaptic bouton preparation / electrophysiological experiment / / / / |
| Reference Info. |
IEICE Tech. Rep., vol. 105, no. 577, MBE2005-108, pp. 5-8, Jan. 2006. |
| Paper # |
MBE2005-108 |
| Date of Issue |
2006-01-20 (MBE) |
| ISSN |
Print edition: ISSN 0913-5685 |
| Download PDF |
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| Conference Information |
| Committee |
MBE |
| Conference Date |
2006-01-27 - 2006-01-28 |
| Place (in Japanese) |
(See Japanese page) |
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| Topics (in Japanese) |
(See Japanese page) |
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| Paper Information |
| Registration To |
MBE |
| Conference Code |
2006-01-MBE |
| Language |
Japanese |
| Title (in Japanese) |
(See Japanese page) |
| Sub Title (in Japanese) |
(See Japanese page) |
| Title (in English) |
Application of the synaptic bouton preparation to rat retinal ganglion cells |
| Sub Title (in English) |
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| Keyword(1) |
retina |
| Keyword(2) |
ganglion cell |
| Keyword(3) |
synaptic bouton preparation |
| Keyword(4) |
electrophysiological experiment |
| Keyword(5) |
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| Keyword(6) |
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| 1st Author's Name |
Tamami Motomura |
| 1st Author's Affiliation |
Kumamoto University (Kumamoto Univ.) |
| 2nd Author's Name |
Yuki Hayashida |
| 2nd Author's Affiliation |
Kumamoto University (Kumamoto Univ.) |
| 3rd Author's Name |
Norio Akaike |
| 3rd Author's Affiliation |
Kumamoto Health Science University (Kumamoto Health Science Univ.) |
| 4th Author's Name |
Nobuki Murayama |
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Kumamoto University (Kumamoto Univ.) |
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| Speaker |
Author-1 |
| Date Time |
2006-01-27 10:25:00 |
| Presentation Time |
25 minutes |
| Registration for |
MBE |
| Paper # |
MBE2005-108 |
| Volume (vol) |
vol.105 |
| Number (no) |
no.577 |
| Page |
pp.5-8 |
| #Pages |
4 |
| Date of Issue |
2006-01-20 (MBE) |