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| Presentation | 2009-01-24 11:50 Study on the antimicrobial activity of Hinokitiol examined by colony counting method and intracellular ATP dose assay Xipeng Zhao, Toshitsugu Sugawara, Satoshi Kuroda, Junji Arisawa, Kazuyuki Kimura (Hokkaido Inst. of Tech.)  MBE2008-88 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | In the previous study, we demonstrated that the small amounts of Hinokitiol which isolated from tree oil inhibited bacterial growth effectively. About 50μg/ml (=ppm) of Hinokitiol clearly suppressed either of Escherichia coli and Staphylococcus aureus growth when they were incubated in optimum growth condition like as broth medium, whereas about 100μg/ml (=ppm) of Hinokitiol administration showed bactericidal activity against employed bacteria in same growth condition. In this present study, we conducted the measurement of bacterial metabolic activity using intracellular ATP assay. About 50μg/ml (=ppm) of Hinokitiol did not affect the growth rate of E. coli and Sta.aureus when its were measured by colony forming examination, however, the ATP doses in both of bacterial strains were remarkably decreased by HK administration. This phenomenon indicated that small amount of HK cloud promote the VNC (Viable but not cultured) condition. These result suggested a newly possibility of HK utilization for infection control substance. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | Hinokitiol / ＡＴＰ dose assay / / / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 108, no. 405, MBE2008-88, pp. 61-65, Jan. 2009. | |
| Paper # | MBE2008-88 | |
| Date of Issue | 2009-01-16 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2008-88 |
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| Conference Information | |
| Committee | MBE |
| Conference Date | 2009-01-23 - 2009-01-24 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Saga University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | ME in general |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2009-01-MBE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Study on the antimicrobial activity of Hinokitiol examined by colony counting method and intracellular ATP dose assay |
| Sub Title (in English) | |
| Keyword(1) | Hinokitiol |
| Keyword(2) | ＡＴＰ dose assay |
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| 1st Author's Name | Xipeng Zhao |
| 1st Author's Affiliation | Hokkaido Institute of Technology (Hokkaido Inst. of Tech.) |
| 2nd Author's Name | Toshitsugu Sugawara |
| 2nd Author's Affiliation | Hokkaido Institute of Technology (Hokkaido Inst. of Tech.) |
| 3rd Author's Name | Satoshi Kuroda |
| 3rd Author's Affiliation | Hokkaido Institute of Technology (Hokkaido Inst. of Tech.) |
| 4th Author's Name | Junji Arisawa |
| 4th Author's Affiliation | Hokkaido Institute of Technology (Hokkaido Inst. of Tech.) |
| 5th Author's Name | Kazuyuki Kimura |
| 5th Author's Affiliation | Hokkaido Institute of Technology (Hokkaido Inst. of Tech.) |
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| Speaker | Author-1 |
| Date Time | 2009-01-24 11:50:00 |
| Presentation Time | 25 minutes |
| Registration for | MBE |
| Paper # | MBE2008-88 |
| Volume (vol) | vol.108 |
| Number (no) | no.405 |
| Page | pp.61-65 |
| #Pages | 5 |
| Date of Issue | 2009-01-16 (MBE) |
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