Paper Abstract and Keywords |
Presentation |
2012-05-25 11:55
Detection of change in mechanical properties of a single DNA molecule stretched by dual trap optical tweezers Yoshihiro Murayama, Yusuke Yogiashi, Takuya Iwamoto (Tokyo Univ. of Agri. and Tech.) LQE2012-6 Link to ES Tech. Rep. Archives: LQE2012-6 |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
When DNA is stretched by dual trap optical tweezers, we can obtain not only force but also local spring constant of a single DNA by calculating auto-correlation and cross correlation of the fluctuation of attached beads. To apply the correlation methods to detect DNA-protein interaction without labeling, we investigated the appropriate condition of experimental setup and its ability for detection. We varied laser power and local spring constant of a stretched DNA, k_DNA, was obtained by using the correlation method at each laser power.
The results show that appropriate spring constant of optical tweezers is 4--5 pN/μm for our setup, and we can detect the change of k_DNA with 0.4 pN/μm accuracy, with the time resolution of 1 s. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
optical tweezers / DNA / mechanical properties / correlation function / / / / |
Reference Info. |
IEICE Tech. Rep., vol. 112, no. 62, LQE2012-6, pp. 25-28, May 2012. |
Paper # |
LQE2012-6 |
Date of Issue |
2012-05-18 (LQE) |
ISSN |
Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 |
Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) |
Download PDF |
LQE2012-6 Link to ES Tech. Rep. Archives: LQE2012-6 |
Conference Information |
Committee |
LQE LSJ |
Conference Date |
2012-05-25 - 2012-05-25 |
Place (in Japanese) |
(See Japanese page) |
Place (in English) |
Univ. of Fukui (Bunkyo Campus) |
Topics (in Japanese) |
(See Japanese page) |
Topics (in English) |
Quantum optics, nonlinear optics, ultra-high speed phenomenon, etc. |
Paper Information |
Registration To |
LQE |
Conference Code |
2012-05-LQE-LSJ |
Language |
Japanese |
Title (in Japanese) |
(See Japanese page) |
Sub Title (in Japanese) |
(See Japanese page) |
Title (in English) |
Detection of change in mechanical properties of a single DNA molecule stretched by dual trap optical tweezers |
Sub Title (in English) |
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optical tweezers |
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DNA |
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mechanical properties |
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correlation function |
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1st Author's Name |
Yoshihiro Murayama |
1st Author's Affiliation |
Tokyo University of Agriculture and Technology (Tokyo Univ. of Agri. and Tech.) |
2nd Author's Name |
Yusuke Yogiashi |
2nd Author's Affiliation |
Tokyo University of Agriculture and Technology (Tokyo Univ. of Agri. and Tech.) |
3rd Author's Name |
Takuya Iwamoto |
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Tokyo University of Agriculture and Technology (Tokyo Univ. of Agri. and Tech.) |
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Speaker |
Author-1 |
Date Time |
2012-05-25 11:55:00 |
Presentation Time |
25 minutes |
Registration for |
LQE |
Paper # |
LQE2012-6 |
Volume (vol) |
vol.112 |
Number (no) |
no.62 |
Page |
pp.25-28 |
#Pages |
4 |
Date of Issue |
2012-05-18 (LQE) |
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