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Paper Abstract and Keywords
Presentation 2015-03-03 09:12
Non-invasive quality evaluation of confluent cells by orientation analysis
Kei Sasaki (Yamagata Univ.), Hiroto Sasaki, Atsuki Takahashi, Kei Kanie (Nagoya Univ.), Siu Kang (Yamagata Univ.), Ryuji Kato (Nagoya Univ.), Tetsuya Yuasa (Yamagata Univ.) MI2014-89
Abstract (in Japanese) (See Japanese page) 
(in English) Although regenerative medicine is rapidly evolving toward clinical application in recent years, the fact that no effective technique to non-invasively and objectively monitor the state of cultured cells is existent hampers its realization. For this problem, an image processing/recognition technique using phase-contrast microscopy images acquired non-invasively, which first segments individual cells and then quantitatively evaluates the quality of each cell morphologically, will be a solution. However, for confluent cells it has been ineffective because the confluent cells have no decisive boundary, and then each cell cannot be segmented and characterized by the morphological features. In this research, we devise a method to quantify the state of confluent cells by scoring each pixel based on the collective feature in terms of orientation and then statistically characterizing the heterogeneity of the score distribution throughout an image. We demonstrate the efficacy of the proposed method by classifying actual microscopic images of confluent cells into proper classes using a support vector machine based on the feature derived from the orientation and heterogeneity.
Keyword (in Japanese) (See Japanese page) 
(in English) Confluent cell / Orientation / Heterogeneity / Phase-contrast microscopic image / / / /  
Reference Info. IEICE Tech. Rep., vol. 114, no. 482, MI2014-89, pp. 167-172, March 2015.
Paper # MI2014-89 
Date of Issue 2015-02-23 (MI) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
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All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MI  
Conference Date 2015-03-02 - 2015-03-03 
Place (in Japanese) (See Japanese page) 
Place (in English) Hotel Miyahira 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Creation of multidisciplinary computational anatomy, Forefront of medical image engineering, General topics in medical imaging 
Paper Information
Registration To MI 
Conference Code 2015-03-MI 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Non-invasive quality evaluation of confluent cells by orientation analysis 
Sub Title (in English)  
Keyword(1) Confluent cell  
Keyword(2) Orientation  
Keyword(3) Heterogeneity  
Keyword(4) Phase-contrast microscopic image  
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1st Author's Name Kei Sasaki  
1st Author's Affiliation Yamagata University (Yamagata Univ.)
2nd Author's Name Hiroto Sasaki  
2nd Author's Affiliation Nagoya University (Nagoya Univ.)
3rd Author's Name Atsuki Takahashi  
3rd Author's Affiliation Nagoya University (Nagoya Univ.)
4th Author's Name Kei Kanie  
4th Author's Affiliation Nagoya University (Nagoya Univ.)
5th Author's Name Siu Kang  
5th Author's Affiliation Yamagata University (Yamagata Univ.)
6th Author's Name Ryuji Kato  
6th Author's Affiliation Nagoya University (Nagoya Univ.)
7th Author's Name Tetsuya Yuasa  
7th Author's Affiliation Yamagata University (Yamagata Univ.)
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Speaker Author-1 
Date Time 2015-03-03 09:12:00 
Presentation Time 12 minutes 
Registration for MI 
Paper # MI2014-89 
Volume (vol) vol.114 
Number (no) no.482 
Page pp.167-172 
#Pages
Date of Issue 2015-02-23 (MI) 


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