Paper Abstract and Keywords |
Presentation |
2015-03-03 09:12
Non-invasive quality evaluation of confluent cells by orientation analysis Kei Sasaki (Yamagata Univ.), Hiroto Sasaki, Atsuki Takahashi, Kei Kanie (Nagoya Univ.), Siu Kang (Yamagata Univ.), Ryuji Kato (Nagoya Univ.), Tetsuya Yuasa (Yamagata Univ.) MI2014-89 |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
Although regenerative medicine is rapidly evolving toward clinical application in recent years, the fact that no effective technique to non-invasively and objectively monitor the state of cultured cells is existent hampers its realization. For this problem, an image processing/recognition technique using phase-contrast microscopy images acquired non-invasively, which first segments individual cells and then quantitatively evaluates the quality of each cell morphologically, will be a solution. However, for confluent cells it has been ineffective because the confluent cells have no decisive boundary, and then each cell cannot be segmented and characterized by the morphological features. In this research, we devise a method to quantify the state of confluent cells by scoring each pixel based on the collective feature in terms of orientation and then statistically characterizing the heterogeneity of the score distribution throughout an image. We demonstrate the efficacy of the proposed method by classifying actual microscopic images of confluent cells into proper classes using a support vector machine based on the feature derived from the orientation and heterogeneity. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
Confluent cell / Orientation / Heterogeneity / Phase-contrast microscopic image / / / / |
Reference Info. |
IEICE Tech. Rep., vol. 114, no. 482, MI2014-89, pp. 167-172, March 2015. |
Paper # |
MI2014-89 |
Date of Issue |
2015-02-23 (MI) |
ISSN |
Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 |
Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) |
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MI2014-89 |
Conference Information |
Committee |
MI |
Conference Date |
2015-03-02 - 2015-03-03 |
Place (in Japanese) |
(See Japanese page) |
Place (in English) |
Hotel Miyahira |
Topics (in Japanese) |
(See Japanese page) |
Topics (in English) |
Creation of multidisciplinary computational anatomy, Forefront of medical image engineering, General topics in medical imaging |
Paper Information |
Registration To |
MI |
Conference Code |
2015-03-MI |
Language |
Japanese |
Title (in Japanese) |
(See Japanese page) |
Sub Title (in Japanese) |
(See Japanese page) |
Title (in English) |
Non-invasive quality evaluation of confluent cells by orientation analysis |
Sub Title (in English) |
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Keyword(1) |
Confluent cell |
Keyword(2) |
Orientation |
Keyword(3) |
Heterogeneity |
Keyword(4) |
Phase-contrast microscopic image |
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1st Author's Name |
Kei Sasaki |
1st Author's Affiliation |
Yamagata University (Yamagata Univ.) |
2nd Author's Name |
Hiroto Sasaki |
2nd Author's Affiliation |
Nagoya University (Nagoya Univ.) |
3rd Author's Name |
Atsuki Takahashi |
3rd Author's Affiliation |
Nagoya University (Nagoya Univ.) |
4th Author's Name |
Kei Kanie |
4th Author's Affiliation |
Nagoya University (Nagoya Univ.) |
5th Author's Name |
Siu Kang |
5th Author's Affiliation |
Yamagata University (Yamagata Univ.) |
6th Author's Name |
Ryuji Kato |
6th Author's Affiliation |
Nagoya University (Nagoya Univ.) |
7th Author's Name |
Tetsuya Yuasa |
7th Author's Affiliation |
Yamagata University (Yamagata Univ.) |
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Speaker |
Author-1 |
Date Time |
2015-03-03 09:12:00 |
Presentation Time |
12 minutes |
Registration for |
MI |
Paper # |
MI2014-89 |
Volume (vol) |
vol.114 |
Number (no) |
no.482 |
Page |
pp.167-172 |
#Pages |
6 |
Date of Issue |
2015-02-23 (MI) |
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