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| Presentation | 2006-07-06 13:20 Liquid Phase Immunoassay Utilizing Magnetic Marker and SQUID Takashi Nishimoto, Takashi Matsuda, Hidetoshi Tokumitsu, Tsuyoshi Tanaka, Keiji Enpuku (Kyushu Univ.) Link to ES Tech. Rep. Archives:  SCE2006-19 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | We have developed a liquid phase-immunoassay system utilizing SQUID and magnetic marker. In this system, the magnetic marker was used to detect the biological material called antigen. The magnetic marker was designed to show the remanence, and the remanence field of the markers that bound to the antigens was measured with the SQUID. The measurement was performed in a solution that contains both the bound and free (or unbound) markers, i.e., without using the so-called Bound/Free (BF) separation process. The Brownian rotation of the free markers was used to distinguish the bound markers from the free ones. The spurious signal from the free markers, which was caused by imperfectness of the Brownian rotation, was studied. Using the system, we showed the detection of the biological material called IgE without BF separation. At present, we could detect the IgE down to 7 pg (or 39 atto-mol). Since the sensitivity was limited by the spurious signal from the free makers, the sensitivity will be improved by improving the dispersion of the markers. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | SQUID / Immunoassay / BF separation / Magnetic marker / Brownian rotation / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 106, no. 139, SCE2006-19, pp. 35-40, July 2006. | |
| Paper # | SCE2006-19 | |
| Date of Issue | 2006-06-29 (SCE) | |
| ISSN | Print edition: ISSN 0913-5685 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | Link to ES Tech. Rep. Archives: SCE2006-19 |
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| Conference Information | |
| Committee | SCE |
| Conference Date | 2006-07-06 - 2006-07-06 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | SQUID, etc. |
| Paper Information | |
| Registration To | SCE |
| Conference Code | 2006-07-SCE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Liquid Phase Immunoassay Utilizing Magnetic Marker and SQUID |
| Sub Title (in English) | |
| Keyword(1) | SQUID |
| Keyword(2) | Immunoassay |
| Keyword(3) | BF separation |
| Keyword(4) | Magnetic marker |
| Keyword(5) | Brownian rotation |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Takashi Nishimoto |
| 1st Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 2nd Author's Name | Takashi Matsuda |
| 2nd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 3rd Author's Name | Hidetoshi Tokumitsu |
| 3rd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 4th Author's Name | Tsuyoshi Tanaka |
| 4th Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 5th Author's Name | Keiji Enpuku |
| 5th Author's Affiliation | Kyushu University (Kyushu Univ.) |
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| Speaker | Author-1 |
| Date Time | 2006-07-06 13:20:00 |
| Presentation Time | 25 minutes |
| Registration for | SCE |
| Paper # | SCE2006-19 |
| Volume (vol) | vol.106 |
| Number (no) | no.139 |
| Page | pp.35-40 |
| #Pages | 6 |
| Date of Issue | 2006-06-29 (SCE) |
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