Paper Abstract and Keywords |
Presentation |
2006-07-14 10:55
Development of a flow-through type pH/CO2 sensor system based on ISFET for cellular function Satoshi Mohri, Michihiro Nakamura, Keiji Naruse (Okayama Univ.) |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
We developed a pH/CO2 sensor system using two ion sensitive field effect transistors (ISFETs) to evaluate the metabolic activity of cultured cells. ISFETs were placed in a flow-through chamber with a window to be attached to a glass-plate on which cells were cultured. Measurements were performed with a baseline solution of low buffer capacity. In the quantitative estimation of cellular products, we assumed that cells secreted CO2, lactic acid, and sodium bicarbonate (NaHCO3), and then lactic acid and NaHCO3 proceeded to the neutralization reaction to form sodium lactate and additional CO2. We applied this system to evaluate the metabolic activity of bovine aortic endothelial cells and rabbit aortic smooth muscle cells. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
ISFET / extracellular acidification / cellular respiration / cellular metabolism / / / / |
Reference Info. |
IEICE Tech. Rep., vol. 106, no. 162, MBE2006-29, pp. 1-4, July 2006. |
Paper # |
MBE2006-29 |
Date of Issue |
2006-07-07 (MBE) |
ISSN |
Print edition: ISSN 0913-5685 |
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