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Paper Abstract and Keywords
Presentation 2006-07-14 10:55
Development of a flow-through type pH/CO2 sensor system based on ISFET for cellular function
Satoshi Mohri, Michihiro Nakamura, Keiji Naruse (Okayama Univ.)
Abstract (in Japanese) (See Japanese page) 
(in English) We developed a pH/CO2 sensor system using two ion sensitive field effect transistors (ISFETs) to evaluate the metabolic activity of cultured cells. ISFETs were placed in a flow-through chamber with a window to be attached to a glass-plate on which cells were cultured. Measurements were performed with a baseline solution of low buffer capacity. In the quantitative estimation of cellular products, we assumed that cells secreted CO2, lactic acid, and sodium bicarbonate (NaHCO3), and then lactic acid and NaHCO3 proceeded to the neutralization reaction to form sodium lactate and additional CO2. We applied this system to evaluate the metabolic activity of bovine aortic endothelial cells and rabbit aortic smooth muscle cells.
Keyword (in Japanese) (See Japanese page) 
(in English) ISFET / extracellular acidification / cellular respiration / cellular metabolism / / / /  
Reference Info. IEICE Tech. Rep., vol. 106, no. 162, MBE2006-29, pp. 1-4, July 2006.
Paper # MBE2006-29 
Date of Issue 2006-07-07 (MBE) 
ISSN Print edition: ISSN 0913-5685
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Conference Information
Committee MBE  
Conference Date 2006-07-14 - 2006-07-14 
Place (in Japanese) (See Japanese page) 
Place (in English) Okayama University Medical School 
Topics (in Japanese) (See Japanese page) 
Topics (in English)  
Paper Information
Registration To MBE 
Conference Code 2006-07-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Development of a flow-through type pH/CO2 sensor system based on ISFET for cellular function 
Sub Title (in English)  
Keyword(1) ISFET  
Keyword(2) extracellular acidification  
Keyword(3) cellular respiration  
Keyword(4) cellular metabolism  
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1st Author's Name Satoshi Mohri  
1st Author's Affiliation Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.)
2nd Author's Name Michihiro Nakamura  
2nd Author's Affiliation Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.)
3rd Author's Name Keiji Naruse  
3rd Author's Affiliation Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.)
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Speaker Author-1 
Date Time 2006-07-14 10:55:00 
Presentation Time 25 minutes 
Registration for MBE 
Paper # MBE2006-29 
Volume (vol) vol.106 
Number (no) no.162 
Page pp.1-4 
#Pages
Date of Issue 2006-07-07 (MBE) 


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