| Paper Abstract and Keywords |
| Presentation |
2006-07-14 10:55
Development of a flow-through type pH/CO2 sensor system based on ISFET for cellular function
Satoshi Mohri, Michihiro Nakamura, Keiji Naruse (Okayama Univ.) |
| Abstract |
(in Japanese) |
(See Japanese page) |
| (in English) |
We developed a pH/CO2 sensor system using two ion sensitive field effect transistors (ISFETs) to evaluate the metabolic activity of cultured cells. ISFETs were placed in a flow-through chamber with a window to be attached to a glass-plate on which cells were cultured. Measurements were performed with a baseline solution of low buffer capacity. In the quantitative estimation of cellular products, we assumed that cells secreted CO2, lactic acid, and sodium bicarbonate (NaHCO3), and then lactic acid and NaHCO3 proceeded to the neutralization reaction to form sodium lactate and additional CO2. We applied this system to evaluate the metabolic activity of bovine aortic endothelial cells and rabbit aortic smooth muscle cells. |
| Keyword |
(in Japanese) |
(See Japanese page) |
| (in English) |
ISFET / extracellular acidification / cellular respiration / cellular metabolism / / / / |
| Reference Info. |
IEICE Tech. Rep., vol. 106, no. 162, MBE2006-29, pp. 1-4, July 2006. |
| Paper # |
MBE2006-29 |
| Date of Issue |
2006-07-07 (MBE) |
| ISSN |
Print edition: ISSN 0913-5685 |
| Download PDF |
|
| Conference Information |
| Committee |
MBE |
| Conference Date |
2006-07-14 - 2006-07-14 |
| Place (in Japanese) |
(See Japanese page) |
| Place (in English) |
Okayama University Medical School |
| Topics (in Japanese) |
(See Japanese page) |
| Topics (in English) |
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| Paper Information |
| Registration To |
MBE |
| Conference Code |
2006-07-MBE |
| Language |
Japanese |
| Title (in Japanese) |
(See Japanese page) |
| Sub Title (in Japanese) |
(See Japanese page) |
| Title (in English) |
Development of a flow-through type pH/CO2 sensor system based on ISFET for cellular function |
| Sub Title (in English) |
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| Keyword(1) |
ISFET |
| Keyword(2) |
extracellular acidification |
| Keyword(3) |
cellular respiration |
| Keyword(4) |
cellular metabolism |
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| 1st Author's Name |
Satoshi Mohri |
| 1st Author's Affiliation |
Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.) |
| 2nd Author's Name |
Michihiro Nakamura |
| 2nd Author's Affiliation |
Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.) |
| 3rd Author's Name |
Keiji Naruse |
| 3rd Author's Affiliation |
Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences (Okayama Univ.) |
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| Speaker |
Author-1 |
| Date Time |
2006-07-14 10:55:00 |
| Presentation Time |
25 minutes |
| Registration for |
MBE |
| Paper # |
MBE2006-29 |
| Volume (vol) |
vol.106 |
| Number (no) |
no.162 |
| Page |
pp.1-4 |
| #Pages |
4 |
| Date of Issue |
2006-07-07 (MBE) |