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Paper Abstract and Keywords
Presentation 2007-10-26 15:05
Effect of OH radicals induced by 1 MHz-ultrasound on HeLa cells
Hiromi Shida, Wakako Hiraoka, Pak-Kon Choi (Meiji Univ.) US2007-73
Abstract (in Japanese) (See Japanese page) 
(in English) We investigated the effects of ultrasound at the frequency of 1 MHz on the adhesive property of HeLa cells which are typical cancer cells, and on the production of hydroxyl radicals in the HeLa cells. These cells are suitable for observation under microscope since they easily adhere to petri dish. The limiting amplitudes of ultrasonic pressure over which the cells were detached were determined in a continuous-wave mode and pulsed-wave mode with the duty ratio of 1:10 in phosphate buffered saline solution. In the case of continuous-wave mode, the cells were detached above the pressure of 0.26 MPa and 0.56 MPa in the air-saturated solution and in the degassed solution, respectively. To examine this difference in the limiting pressure amplitude, we evaluated the production of hydroxyl radicals by KI colorimetric procedure. The quantities of the hydroxyl radicals in the degassed solution and in the air-saturated solution were approximately equal. This result indicates that hydroxyl radicals are not the major cause of the cell detachment. The number of cavitation bubbles is considerably large in air-saturated solution and bubbles existing near solid boundary may produce jet impact to the cells. To study the production of hydroxyl radicals in HeLa cells, we employed aminophenyl fluorescein which reacts with hydroxyl radicals and emits light of 515 nm after exciting with light of 488 nm. We observed fluorescence from the cells under microscope after the ultrasound at the pressure of 0.56 MPa was irradiated in degassed solution. If the fluorescence comes from inside of the cells, we can say that hydroxyl radicals are produced inside of the cells. However, we need to check that the fluorescence does not come from the surface of the cells where aminophenyl fluorescein may be adsorbed.
Keyword (in Japanese) (See Japanese page) 
(in English) fluorescence / aminophenyl fluorescein / HeLa cells / hydroxyl radicals / ultrasound / / /  
Reference Info. IEICE Tech. Rep., vol. 107, no. 284, US2007-73, pp. 39-43, Oct. 2007.
Paper # US2007-73 
Date of Issue 2007-10-19 (US) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee US  
Conference Date 2007-10-25 - 2007-10-26 
Place (in Japanese) (See Japanese page) 
Place (in English) Meiji Univ. (Akihabara) 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Sono-Physics, General 
Paper Information
Registration To US 
Conference Code 2007-10-US 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Effect of OH radicals induced by 1 MHz-ultrasound on HeLa cells 
Sub Title (in English)  
Keyword(1) fluorescence  
Keyword(2) aminophenyl fluorescein  
Keyword(3) HeLa cells  
Keyword(4) hydroxyl radicals  
Keyword(5) ultrasound  
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Keyword(8)  
1st Author's Name Hiromi Shida  
1st Author's Affiliation Meiji University (Meiji Univ.)
2nd Author's Name Wakako Hiraoka  
2nd Author's Affiliation Meiji University (Meiji Univ.)
3rd Author's Name Pak-Kon Choi  
3rd Author's Affiliation Meiji University (Meiji Univ.)
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Speaker Author-1 
Date Time 2007-10-26 15:05:00 
Presentation Time 20 minutes 
Registration for US 
Paper # US2007-73 
Volume (vol) vol.107 
Number (no) no.284 
Page pp.39-43 
#Pages 5 
Date of Issue 2007-10-19 (US) 

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