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| Presentation | 2007-11-28 15:00 Label-free detection of protein using a metallic mesh Hisa Yoshida, Yasuhide Kawai (Tohoku Univ.), Shin'ichiro Hayashi (RIKEN), Yuichi Ogawa (Tohoku Univ.)  ED2007-205 Link to ES Tech. Rep. Archives: ED2007-205 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | We propose a new label-free sensor of protein using a metallic mesh in THz region. Our sensing method relies on a change in the transmittance of THz radiation passed through the metallic mesh on which a sample substance is contacted. By using this technique, we demonstrated the highly sensitive detection the protein horseradish peroxidase, which is an oxidation/reduction enzyme that exists in many organisms. For quantitative investigation of the sensitivity of our sensor, the enzyme was printed on surface of the metallic mesh by a commercial printer. A distinct shift in the transmittance spectrum toward lower frequency was observed for 500 pg/mm2(11 fmol) enzyme printed on the metallic mesh. Next we demonstrated to detect a streptavidin-biotin interaction on a PVDF membrane which is generally used fixation of protein. The streptavidin-biotin interaction can be detected by using 650 pg/mm2 (2.5 pmol) biotin.These results indicate that our sensing system has high sensitivity and we have successfully demonstrated a convenient sensing system by using a metallic mesh and a PVDF membrane. |
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| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | label-free / metallic mesh / membrane / / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 107, no. 355, ED2007-205, pp. 99-102, Nov. 2007. | |
| Paper # | ED2007-205 | |
| Date of Issue | 2007-11-20 (ED) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | ED2007-205 Link to ES Tech. Rep. Archives: ED2007-205 |
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| Conference Information | |
| Committee | ED |
| Conference Date | 2007-11-27 - 2007-11-28 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tohoku Univ. Research Institute of Electrical Communication |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | ED |
| Conference Code | 2007-11-ED |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Label-free detection of protein using a metallic mesh |
| Sub Title (in English) | |
| Keyword(1) | label-free |
| Keyword(2) | metallic mesh |
| Keyword(3) | membrane |
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| 1st Author's Name | Hisa Yoshida |
| 1st Author's Affiliation | Tohoku University (Tohoku Univ.) |
| 2nd Author's Name | Yasuhide Kawai |
| 2nd Author's Affiliation | Tohoku University (Tohoku Univ.) |
| 3rd Author's Name | Shin'ichiro Hayashi |
| 3rd Author's Affiliation | RIKEN (RIKEN) |
| 4th Author's Name | Yuichi Ogawa |
| 4th Author's Affiliation | Tohoku University (Tohoku Univ.) |
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| Speaker | Author-1 |
| Date Time | 2007-11-28 15:00:00 |
| Presentation Time | 25 minutes |
| Registration for | ED |
| Paper # | ED2007-205 |
| Volume (vol) | vol.107 |
| Number (no) | no.355 |
| Page | pp.99-102 |
| #Pages | 4 |
| Date of Issue | 2007-11-20 (ED) |
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