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Paper Abstract and Keywords
Presentation 2008-01-25 15:30
[Poster Presentation] Ca2+ channel subtypes in signle synaptic boutons
Kiyomitsu Shoudai (Kumamoto Univ. / Kumamoto Health Science Univ.), Nobuki Murayama (Kumamoto Univ.) MBE2007-92
Abstract (in Japanese) (See Japanese page) 
(in English) Ca2+ channels play pivotal roles for neuronal functions. The precise distribution of the high-voltage activated (HVA) calcium channel subtypes (L-, N-, P-, Q-, R- types) and low-voltage activated (LVA or T-type) calcium channel in neuronal soma membrane has been confirmed by using immunohistochemistry as well as electrophysiological approaches. Some calcium channel subtypes such as P-type were found to be linked mainly to dendrite localisation. However, the functional specificities of the subtypes which distribute on the small nerve endings (Φ: less than 1μm) are not known so far. In the present study, the single neurons mechanically dissociated from the sacral commissural nucleus (SDCN) of rat (7-15 days old) spinal cord were used. These single neurons, namely ‘synaptic bouton’ preparations, are attached with many functional nerve terminals. One of single boutons was selectively stimulated (5-10μA,0.2 Hz,100μsec of duration) by using ‘focal stimulation’ technique. The evoked inhibitory glycinergic postsynaptic currents (eIPSCs) were isolated pharmacologically by blocking the glutamatergic and GABAergic inputs with CNQX/D-AP5 and bicuculline, respectively. Successively, specific HVA and LVA Ca2+ channel subtype blockers (ω-con GVIA, ω-Aga IVA, nifedipine, efonidipine) and non- selective blocker were examined on glycinergic eIPSCs to distinguish each HVA and LVA Ca2+ channel subtypes. In addition, obtained results were compared with those of HVA Ca2+ channel subtypes distribution of GABAergic nerve endings on hippocampus CA1 neuron.
Keyword (in Japanese) (See Japanese page) 
(in English) Ca2+ channels / nerve endings / glycinergic / IPSC / focal stimulation / SDCN / mechanically dissociation /  
Reference Info. IEICE Tech. Rep., vol. 107, no. 460, MBE2007-92, pp. 51-54, Jan. 2008.
Paper # MBE2007-92 
Date of Issue 2008-01-18 (MBE) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
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reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MBE  
Conference Date 2008-01-25 - 2008-01-25 
Place (in Japanese) (See Japanese page) 
Place (in English) Kyushu Univ. 
Topics (in Japanese) (See Japanese page) 
Topics (in English)  
Paper Information
Registration To MBE 
Conference Code 2008-01-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Ca2+ channel subtypes in signle synaptic boutons 
Sub Title (in English)  
Keyword(1) Ca2+ channels  
Keyword(2) nerve endings  
Keyword(3) glycinergic  
Keyword(4) IPSC  
Keyword(5) focal stimulation  
Keyword(6) SDCN  
Keyword(7) mechanically dissociation  
Keyword(8)  
1st Author's Name Kiyomitsu Shoudai  
1st Author's Affiliation Kumamoto University / Kumamoto Health Science University (Kumamoto Univ. / Kumamoto Health Science Univ.)
2nd Author's Name Nobuki Murayama  
2nd Author's Affiliation Kumamoto University (Kumamoto Univ.)
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Speaker Author-1 
Date Time 2008-01-25 15:30:00 
Presentation Time 5 minutes 
Registration for MBE 
Paper # MBE2007-92 
Volume (vol) vol.107 
Number (no) no.460 
Page pp.51-54 
#Pages 4 
Date of Issue 2008-01-18 (MBE) 

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