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Paper Abstract and Keywords
Presentation 2010-03-09 14:10
Mathematical analysis of space-clamp problems in voltage-clamp recording.
Hiromu Monai, Toru Aonishi (Tokyo Inst. of Tech.) NC2009-95
Abstract (in Japanese) (See Japanese page) 
(in English) The measurement of synaptic current at the distal dendrite with the somatic voltage clamp has an error due to a failure of space clamp in the whole neuron.
Williams \& Mitchell quantified space-clamp errors by using the simultaneous recording from the soma and apical dendrite of rat cortical layer V pyramidal neurons.
It has been reported that the electrotonic length of cortical layer V pyramidal neuron is about 1.
Contrary to this, Williams \& Mitchell showed the space-clamp error is larger than that of the electric cable with electrotonic length 1.
Recent experimental and theoretical studies have suggested that the membrane resistivity of the distal apical dendrites of cortical layer V pyramidal neurons may be significantly lower than that of the proximal dendrites and the soma.
Therefore, it is possible that the large space-clamp error is due to the low membrane resistivity at distal apical dendrites.
In this paper, we formulate an electric behavior of the apical dendrite with the leaky distal part when clamping the somatic membrane potential as a finite passive cable equation with the fixed and leaky boundary conditions, and by employing Green function method we elucidate the effect of the low membrane resistivity at distal apical dendrites on the space-clamp error.
The solutions for a cable with a leak at one end qualitatively reproduce the space-clamp errors measured by Williams \& Mitchell, whereas those without the leak cannot reproduce them even its electrotonic length is adjusted.
Keyword (in Japanese) (See Japanese page) 
(in English) voltage-clamp recording / space-clamp probrems / cable equation / dynamic-clamp / / / /  
Reference Info. IEICE Tech. Rep., vol. 109, no. 461, NC2009-95, pp. 45-50, March 2010.
Paper # NC2009-95 
Date of Issue 2010-03-02 (NC) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF NC2009-95

Conference Information
Committee NC MBE  
Conference Date 2010-03-09 - 2010-03-11 
Place (in Japanese) (See Japanese page) 
Place (in English) Tamagawa University 
Topics (in Japanese) (See Japanese page) 
Topics (in English) General 
Paper Information
Registration To NC 
Conference Code 2010-03-NC-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Mathematical analysis of space-clamp problems in voltage-clamp recording. 
Sub Title (in English)  
Keyword(1) voltage-clamp recording  
Keyword(2) space-clamp probrems  
Keyword(3) cable equation  
Keyword(4) dynamic-clamp  
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1st Author's Name Hiromu Monai  
1st Author's Affiliation Tokyo Institute of Technology (Tokyo Inst. of Tech.)
2nd Author's Name Toru Aonishi  
2nd Author's Affiliation Tokyo Institute of Technology (Tokyo Inst. of Tech.)
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Speaker Author-1 
Date Time 2010-03-09 14:10:00 
Presentation Time 25 minutes 
Registration for NC 
Paper # NC2009-95 
Volume (vol) vol.109 
Number (no) no.461 
Page pp.45-50 
#Pages 6 
Date of Issue 2010-03-02 (NC) 

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