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| Presentation | 2010-05-27 15:20 Protein Patterning Technique with Partial Plasma Treatment Takahiro Ii, Akira Irie, Hayato Takahashi, Hitoshi Muguruma (Shibaura Inst. of Tech.)  OME2010-23 Link to ES Tech. Rep. Archives: OME2010-23 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | Techniques for patterned modification of substrate surfaces are important for forming microarrays on protein chips. The strategy is based on a partial plasma polymerization for coexistence of protein adhesive/non-adhesive region in several ten micrometer size. The best combination of plasma polymerized films (PPFs) was the monomers of hexamethyldisiloxane (HMDS) and tetraethyleneglycoldiethylether (TEGDE) from the points of contrast, resolution, and reproducibility. When surface adsorption of an a protein was visualized by fluorescence microscopy, distinct 80×80 m2 square spots were observed, surrounded by a non-fluorescent 80 m-wide grid. This pattern could be attributed to proteins selectively adsorbed onto the HMDS PPF surface but not onto the surface of TEGDM PPF. The adsorbed antibody proteins selectively binded to the counterpart of the protein and did not do to the non-targeted proteins, demonstrating that the proteins adsorbed onto the PPF surface while not only retaining the biofunctional activity but also providing the suitable orientation of the molecule. This provided a simple fabrication method of protein patterning. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | Protein chip / patterning / plasma polymerization / / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 110, no. 59, OME2010-23, pp. 31-35, May 2010. | |
| Paper # | OME2010-23 | |
| Date of Issue | 2010-05-20 (OME) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | OME2010-23 Link to ES Tech. Rep. Archives: OME2010-23 |
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| Conference Information | |
| Committee | OME |
| Conference Date | 2010-05-27 - 2010-05-27 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | Organic Materials, General |
| Paper Information | |
| Registration To | OME |
| Conference Code | 2010-05-OME |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Protein Patterning Technique with Partial Plasma Treatment |
| Sub Title (in English) | |
| Keyword(1) | Protein chip |
| Keyword(2) | patterning |
| Keyword(3) | plasma polymerization |
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| 1st Author's Name | Takahiro Ii |
| 1st Author's Affiliation | Shibaura Institute of Technology (Shibaura Inst. of Tech.) |
| 2nd Author's Name | Akira Irie |
| 2nd Author's Affiliation | Shibaura Institute of Technology (Shibaura Inst. of Tech.) |
| 3rd Author's Name | Hayato Takahashi |
| 3rd Author's Affiliation | Shibaura Institute of Technology (Shibaura Inst. of Tech.) |
| 4th Author's Name | Hitoshi Muguruma |
| 4th Author's Affiliation | Shibaura Institute of Technology (Shibaura Inst. of Tech.) |
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| Speaker | Author-1 |
| Date Time | 2010-05-27 15:20:00 |
| Presentation Time | 20 minutes |
| Registration for | OME |
| Paper # | OME2010-23 |
| Volume (vol) | vol.110 |
| Number (no) | no.59 |
| Page | pp.31-35 |
| #Pages | 5 |
| Date of Issue | 2010-05-20 (OME) |
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