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Paper Abstract and Keywords
Presentation 2010-06-30 16:15
Focal photolysis of caged glutamate in neuron/glia co-cultures for a new in vitro model of brain ischemia
Tomoharu Watanabe, Sadahiro Iwabuchi, Koichi Kawahara (Hokkaido Univ) MBE2010-15
Abstract (in Japanese) (See Japanese page) 
(in English) Glutamate is released from injured neurons and astrocytes caused by severe ischemic stress. A marked elevation in the concentration of extracellular glutamate (Glu) induces excessive neuronal depolarization, resulting in acute neuronal death in the ischemic core region. In addition, Glu diffused from the ischemic core possibly induces delayed neuronal death in the surroundings of the core region (ischemic penumbra). Here we developed a new in vitro model of brain ischemia by focal photolysis of caged glutamate. A caged glutamate is one of the “caged compounds” activated by irradiation with a pulse of ultraviolet light. This method makes it possible to increase the concentration of extracellular Glu in the focal flashed area. Focal photolysis of a caged Glu resulted in the induction of acute neuronal death in the photolytic-flashed area (FA) and delayed neuronal death in the region surrounding the FA (SFA) in neuron/glia co-cultures.
Keyword (in Japanese) (See Japanese page) 
(in English) ischemia / neuron / ischemic tolerance / delayed neuronal death / caged glutamate / / /  
Reference Info. IEICE Tech. Rep., vol. 110, no. 111, MBE2010-15, pp. 23-26, June 2010.
Paper # MBE2010-15 
Date of Issue 2010-06-23 (MBE) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
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reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MBE  
Conference Date 2010-06-30 - 2010-06-30 
Place (in Japanese) (See Japanese page) 
Place (in English) Hokkaido Univ. 
Topics (in Japanese) (See Japanese page) 
Topics (in English) ME in general 
Paper Information
Registration To MBE 
Conference Code 2010-06-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Focal photolysis of caged glutamate in neuron/glia co-cultures for a new in vitro model of brain ischemia 
Sub Title (in English)  
Keyword(1) ischemia  
Keyword(2) neuron  
Keyword(3) ischemic tolerance  
Keyword(4) delayed neuronal death  
Keyword(5) caged glutamate  
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1st Author's Name Tomoharu Watanabe  
1st Author's Affiliation Hokkaido University (Hokkaido Univ)
2nd Author's Name Sadahiro Iwabuchi  
2nd Author's Affiliation Hokkaido University (Hokkaido Univ)
3rd Author's Name Koichi Kawahara  
3rd Author's Affiliation Hokkaido University (Hokkaido Univ)
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Speaker Author-1 
Date Time 2010-06-30 16:15:00 
Presentation Time 25 minutes 
Registration for MBE 
Paper # MBE2010-15 
Volume (vol) vol.110 
Number (no) no.111 
Page pp.23-26 
#Pages 4 
Date of Issue 2010-06-23 (MBE) 

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