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Paper Abstract and Keywords
Presentation 2012-12-05 14:40
Gene expression analysis of cancer cells using magnetically controlled on chip system
Mina Okochi (Nagoya Univ.) OME2012-78
Abstract (in Japanese) (See Japanese page) 
(in English) Microengineering techniques such as lab-on-a-chip technologies are emerging as the next revolution in tools for high throughput cell-based analysis. Since single-cell analysis enables to capture the minimal change within a living organism, it is emerging as less-invasive approach for clinical diagnosis and cell identification. In this study, we developed technologies using magnetic nanoparticles for cellular analysis of individual cells, such as magnetic force-based cell patterning and droplet-based gene expression analysis. A droplet manipulation system using superparamagnetic nanoparticles and magnetic force was developed for gene expression analysis at single cell levels. The magnetic force-based-droplet handling system can be used as a powerful tool for various biochemical applications by moving and coalescing sample droplets using magnetic beads immersed in mineral oil. We optimized the conditions for single cell lysis and RT-PCR analysis and applied to detection of gastric cancer cells in peritoneal washing, that is useful for prediction of cancer recurrence. The developed system is quite simple and allows for the handling of droplets and preceding reactions using a magnet without external pumps and valves. These systems are greatly advantageous for cell analysis and related diagnostic applications.
Keyword (in Japanese) (See Japanese page) 
(in English) magnetic particles / cancer cells / droplet / gene expression analysis / cell array / / /  
Reference Info. IEICE Tech. Rep., vol. 112, no. 333, OME2012-78, pp. 19-22, Dec. 2012.
Paper # OME2012-78 
Date of Issue 2012-11-28 (OME) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
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reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee OME  
Conference Date 2012-12-05 - 2012-12-05 
Place (in Japanese) (See Japanese page) 
Place (in English) Okinawa-seinen-kaikan 
Topics (in Japanese) (See Japanese page) 
Topics (in English) baiotechnology, solid/liquid interface, materials. electron transfer reaction, etc. 
Paper Information
Registration To OME 
Conference Code 2012-12-OME 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Gene expression analysis of cancer cells using magnetically controlled on chip system 
Sub Title (in English)  
Keyword(1) magnetic particles  
Keyword(2) cancer cells  
Keyword(3) droplet  
Keyword(4) gene expression analysis  
Keyword(5) cell array  
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1st Author's Name Mina Okochi  
1st Author's Affiliation Nagoya University (Nagoya Univ.)
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Speaker Author-1 
Date Time 2012-12-05 14:40:00 
Presentation Time 25 minutes 
Registration for OME 
Paper # OME2012-78 
Volume (vol) vol.112 
Number (no) no.333 
Page pp.19-22 
#Pages 4 
Date of Issue 2012-11-28 (OME) 

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