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Paper Abstract and Keywords
Presentation 2013-04-26 15:25
Electrochemical measurement of alkaline phosphatase activity to evaluate developmental potential of mouse embryonic stem (ES) cells
Hitoshi Shiku, Toshiharu Arai, Taku Nishijo, Yuanshu Zhou, Kosuke Ino, Tomokazu Matsue (Tohoku Univ.) SDM2013-20 OME2013-20
Abstract (in Japanese) (See Japanese page) 
(in English) Electrochemical imaging techniques including scanning electrochemical microscopy (SECM) and local redox cycling-based electrochemical (LRC-EC) chip device have been applied for quantitative evaluation of alkaline phosphatase (ALP) activity of mouse embryonic stem (ES) cells to judge the state of undifferentiation of the cellular samples. Our techniques allowed rapid and high-throughput measuring procedures for ALP activity of stem cells which remarkably improved the invasiveness against the undifferentiated cellular samples. The ES cells and embryoid bodies (EBs) that have been used for ALP assay were found to be re-usable for further cultivation to induce differentiation into cardiomyocyte-like cells. We also mention about background researches done by us and other groups including non-invasive measurement of respiration activity of embryos and EBs for quality control.
Keyword (in Japanese) (See Japanese page) 
(in English) Embryonic stem (ES) cell / Embryoid body (EB) / Differentiation / Alkaline phosphatase / Non-invasive measuring / Electrochemical imaging / /  
Reference Info. IEICE Tech. Rep., vol. 113, no. 18, OME2013-20, pp. 93-96, April 2013.
Paper # OME2013-20 
Date of Issue 2013-04-18 (SDM, OME) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF SDM2013-20 OME2013-20

Conference Information
Committee OME SDM  
Conference Date 2013-04-25 - 2013-04-26 
Place (in Japanese) (See Japanese page) 
Place (in English) Yakusima Environmental Culture Village Center 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Advanced Thin-Film Devices (Si, Compound, Organic), biotechnology, Materials, Measurement Technique and Related Topics 
Paper Information
Registration To OME 
Conference Code 2013-04-OME-SDM 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Electrochemical measurement of alkaline phosphatase activity to evaluate developmental potential of mouse embryonic stem (ES) cells 
Sub Title (in English)  
Keyword(1) Embryonic stem (ES) cell  
Keyword(2) Embryoid body (EB)  
Keyword(3) Differentiation  
Keyword(4) Alkaline phosphatase  
Keyword(5) Non-invasive measuring  
Keyword(6) Electrochemical imaging  
Keyword(7)  
Keyword(8)  
1st Author's Name Hitoshi Shiku  
1st Author's Affiliation Tohoku University (Tohoku Univ.)
2nd Author's Name Toshiharu Arai  
2nd Author's Affiliation Tohoku University (Tohoku Univ.)
3rd Author's Name Taku Nishijo  
3rd Author's Affiliation Tohoku University (Tohoku Univ.)
4th Author's Name Yuanshu Zhou  
4th Author's Affiliation Tohoku University (Tohoku Univ.)
5th Author's Name Kosuke Ino  
5th Author's Affiliation Tohoku University (Tohoku Univ.)
6th Author's Name Tomokazu Matsue  
6th Author's Affiliation Tohoku University (Tohoku Univ.)
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Speaker Author-1 
Date Time 2013-04-26 15:25:00 
Presentation Time 25 minutes 
Registration for OME 
Paper # SDM2013-20, OME2013-20 
Volume (vol) vol.113 
Number (no) no.17(SDM), no.18(OME) 
Page pp.93-96 
#Pages 4 
Date of Issue 2013-04-18 (SDM, OME) 

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