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| Presentation | 2014-01-25 11:45 Multiplex detection of DNA and RNA viruses using RT-LAMP and metal coated hollow fiber membrane Ryo Oi, Toshitsugu Sugawara, Satoshi Kuroda, Junji Arisawa, Kazuyuki Kimura (Hokkaido Inst. of Tech.)  MBE2013-110 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | We proposed a gene amplification method that enables the detection against small amount of viruses. It is intended for application to nucleic acid amplification test (NAT) that is performed on a subject plasma samples for the Japanese Red Cross Society to ensure the safety of the blood supply. A metal coated hollow fiber membrane was used for detection of virus. The membrane was able to isolate nucleic acids after concentration of virus. Isolated nucleic acids were applied to LAMP(Loop-Mediated Isothermal Amplification). This method was rapid and sensitive detection. In this report, to detect DNA and RNA viruses in one step, we tried to apply nucleic acids of DNA or RNA virus in one step. RT-LAMP could also detect DNA or RNA virus in one step. As a result, be detectable both DNA and RNA viruses also stable were found. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | blood supply / metal-coated hollow fiber membrane / RT-LAMP / Multiplex detection / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 113, no. 409, MBE2013-110, pp. 79-83, Jan. 2014. | |
| Paper # | MBE2013-110 | |
| Date of Issue | 2014-01-17 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2013-110 |
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| Conference Information | |
| Committee | MBE |
| Conference Date | 2014-01-24 - 2014-01-25 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Saga University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2014-01-MBE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Multiplex detection of DNA and RNA viruses using RT-LAMP and metal coated hollow fiber membrane |
| Sub Title (in English) | |
| Keyword(1) | blood supply |
| Keyword(2) | metal-coated hollow fiber membrane |
| Keyword(3) | RT-LAMP |
| Keyword(4) | Multiplex detection |
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| 1st Author's Name | Ryo Oi |
| 1st Author's Affiliation | Hokkaido Institute of technology (Hokkaido Inst. of Tech.) |
| 2nd Author's Name | Toshitsugu Sugawara |
| 2nd Author's Affiliation | Hokkaido Institute of technology (Hokkaido Inst. of Tech.) |
| 3rd Author's Name | Satoshi Kuroda |
| 3rd Author's Affiliation | Hokkaido Institute of technology (Hokkaido Inst. of Tech.) |
| 4th Author's Name | Junji Arisawa |
| 4th Author's Affiliation | Hokkaido Institute of technology (Hokkaido Inst. of Tech.) |
| 5th Author's Name | Kazuyuki Kimura |
| 5th Author's Affiliation | Hokkaido Institute of technology (Hokkaido Inst. of Tech.) |
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| Speaker | Author-1 |
| Date Time | 2014-01-25 11:45:00 |
| Presentation Time | 25 minutes |
| Registration for | MBE |
| Paper # | MBE2013-110 |
| Volume (vol) | vol.113 |
| Number (no) | no.409 |
| Page | pp.79-83 |
| #Pages | 5 |
| Date of Issue | 2014-01-17 (MBE) |
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