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| Presentation | 2014-12-13 10:00 Simple and Rapid Renal Function Analysis Using Newly Conducted Enzyme-Colorimetric Microassay (2) -- Determination of Uric Acid and Creatinine -- Toru Yokoyama (Sapporo Coll. of Med., Welfare and Dent. Tech.)  MBE2014-76 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | The author describes newly conducted quantitative and sensitive microassays for uric acid (UA) and creatinine determination, using uricase-quinoneimine-dye and creatinine-deiminase-indophenol methods. This UA assay uses the catalyzed quinoneimine dye reaction for the determination of hydrogen peroxide. The procedure was adapted for the determination of UA after oxidization with uricase. The final product in this reaction, which is the maximum purple color of quinoneimine dye, required 5 min at 37 °C or 10 min at room temperature, and absorbance was measured at 570 nm using a microplate reader. This creatinine assay uses the catalyzed indophenol reaction for the determination of ammonia. The blue indophenol as the final product required 10 min at 60 °C. We demonstrated that this convenient colorimetric microplate-based rapid assay can be applied to determine UA and creatinine as an evaluation of renal function in biological fluids, such as urine, serum or plasma, and spent dialysate, during hemodialysis. This rapid and reliable assay system is very useful as the primary screening of urinalysis. In this study, the author has developed the novel microassays to determine urea which reported previously, UA and creatinine as typical three test items for renal function evaluation. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | microplate-based assay / uric acid / creatinine / enzyme-colorimetric method / renal function / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 114, no. 361, MBE2014-76, pp. 11-16, Dec. 2014. | |
| Paper # | MBE2014-76 | |
| Date of Issue | 2014-12-06 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2014-76 |
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| Conference Information | |
| Committee | MBE NC |
| Conference Date | 2014-12-13 - 2014-12-13 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Nagoya University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2014-12-MBE-NC |
| Language | English |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Simple and Rapid Renal Function Analysis Using Newly Conducted Enzyme-Colorimetric Microassay (2) |
| Sub Title (in English) | Determination of Uric Acid and Creatinine |
| Keyword(1) | microplate-based assay |
| Keyword(2) | uric acid |
| Keyword(3) | creatinine |
| Keyword(4) | enzyme-colorimetric method |
| Keyword(5) | renal function |
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| 1st Author's Name | Toru Yokoyama |
| 1st Author's Affiliation | Sapporo College of Medical, Welfare and Dental Technology (Sapporo Coll. of Med., Welfare and Dent. Tech.) |
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| Speaker | Author-1 |
| Date Time | 2014-12-13 10:00:00 |
| Presentation Time | 20 minutes |
| Registration for | MBE |
| Paper # | MBE2014-76 |
| Volume (vol) | vol.114 |
| Number (no) | no.361 |
| Page | pp.11-16 |
| #Pages | 6 |
| Date of Issue | 2014-12-06 (MBE) |
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