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Paper Abstract and Keywords
Presentation 2015-03-16 11:25
Development and evaluation of micro cell culture wells for studies on efficient sonoporation
You Shirahata, Kaori Kuribayasi-Shigetomi, Nobuki Kudo (Hokkaido Univ.) MBE2014-119 NC2014-70
Abstract (in Japanese) (See Japanese page) 
(in English) We have been studying a sonoporation technique that transduces drugs into cells by exposure of the cells with adjacent bubbles to short-pulsed ultrasound. In the research process, it was found that efficiency of the cell membrane repair after sonoporation is affected by the cell membrane tension. In this study, micron-sized wells that can culture several tens of cells were designed and fabricated to control cell membrane tension by changing extension state of the cells. The wells were manufactured using a conventional photolithography technique with thick photoresist SU-8. The wells are designed to be 15－200 µm in diameter and about 40 µm in depth. The developed wells were used for 1-week cell culture, and it was confirmed that adherent areas of cells can be controlled by controlling number of the cells cultured inside a well, indicating that the developed wells are useful tools for future studies on in vivo sonoporation.
Keyword (in Japanese) (See Japanese page) 
(in English) sonoporation / ultrasound / microbubble / photolithography / SU-8 / / /  
Reference Info. IEICE Tech. Rep., vol. 114, no. 514, MBE2014-119, pp. 19-23, March 2015.
Paper # MBE2014-119 
Date of Issue 2015-03-09 (MBE, NC) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF MBE2014-119 NC2014-70

Conference Information
Committee NC MBE  
Conference Date 2015-03-16 - 2015-03-17 
Place (in Japanese) (See Japanese page) 
Place (in English) Tamagawa University 
Topics (in Japanese) (See Japanese page) 
Topics (in English) ME, etc 
Paper Information
Registration To MBE 
Conference Code 2015-03-NC-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Development and evaluation of micro cell culture wells for studies on efficient sonoporation 
Sub Title (in English)  
Keyword(1) sonoporation  
Keyword(2) ultrasound  
Keyword(3) microbubble  
Keyword(4) photolithography  
Keyword(5) SU-8  
Keyword(6)  
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Keyword(8)  
1st Author's Name You Shirahata  
1st Author's Affiliation Hokkaido University (Hokkaido Univ.)
2nd Author's Name Kaori Kuribayasi-Shigetomi  
2nd Author's Affiliation Hokkaido University (Hokkaido Univ.)
3rd Author's Name Nobuki Kudo  
3rd Author's Affiliation Hokkaido University (Hokkaido Univ.)
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Speaker Author-1 
Date Time 2015-03-16 11:25:00 
Presentation Time 25 minutes 
Registration for MBE 
Paper # MBE2014-119, NC2014-70 
Volume (vol) vol.114 
Number (no) no.514(MBE), no.515(NC) 
Page pp.19-23 
#Pages 5 
Date of Issue 2015-03-09 (MBE, NC) 

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