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Paper Abstract and Keywords
Presentation 2015-04-29 13:40
Coordination chemistry of active center of metalloenzyme studied by affinity capillary electrophoresis
Nobuhiko Iki, Yosuke Sato (Tohoku Univ.) SDM2015-3 OME2015-3
Abstract (in Japanese) (See Japanese page) 
(in English) By using affinity capillary electrophoresis, binding equilibrium of an arylsulfonamide (SA) to a series of metal-substituted carbonic anhydrase (M-CA) and kinetic stability of M-CA toward solvolysis were studied. In the binding of SA, the hydrophobic cavity of CA plays an important role to force the SA to donate the amide moiety to the metal center of CA to fulfill the tetrahedral coordination geometry. In the kinetics, the hydrophobic cavity excludes the access of EDTA to the metal center, while the tetrahedral coordination geometry supported by three histidine residues gave the Zn(II) center exceptionally high kinetic stability.
Keyword (in Japanese) (See Japanese page) 
(in English) Metalloenzyme / Carbonic Anhydrase / Zinc / Affinity Capillary Electrophoresis / / / /  
Reference Info. IEICE Tech. Rep., vol. 115, no. 19, OME2015-3, pp. 11-14, April 2015.
Paper # OME2015-3 
Date of Issue 2015-04-22 (SDM, OME) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF SDM2015-3 OME2015-3

Conference Information
Committee OME SDM  
Conference Date 2015-04-29 - 2015-04-30 
Place (in Japanese) (See Japanese page) 
Place (in English) Oh-hama Nobumoto Memorial Hall 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Thin FIlms, Functional Electronics Devices, New Functional Materials and Evaluation, BIomtechnology 
Paper Information
Registration To OME 
Conference Code 2015-04-OME-SDM 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Coordination chemistry of active center of metalloenzyme studied by affinity capillary electrophoresis 
Sub Title (in English)  
Keyword(1) Metalloenzyme  
Keyword(2) Carbonic Anhydrase  
Keyword(3) Zinc  
Keyword(4) Affinity Capillary Electrophoresis  
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1st Author's Name Nobuhiko Iki  
1st Author's Affiliation Tohoku University (Tohoku Univ.)
2nd Author's Name Yosuke Sato  
2nd Author's Affiliation Tohoku University (Tohoku Univ.)
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Speaker Author-1 
Date Time 2015-04-29 13:40:00 
Presentation Time 25 minutes 
Registration for OME 
Paper # SDM2015-3, OME2015-3 
Volume (vol) vol.115 
Number (no) no.18(SDM), no.19(OME) 
Page pp.11-14 
#Pages 4 
Date of Issue 2015-04-22 (SDM, OME) 

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