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| Presentation | 2017-01-19 14:50 Effect of reaction conditions on magnetic signal from magnetic markers in liquid-phase immunoassays Kota Nakamura, Yoshitsugu Takafuji, Kota Irie, Teruyoshi Sasayama, Takashi Yoshida, Keiji Enpuku (Kyushu Univ.)  SCE2016-46 Link to ES Tech. Rep. Archives: SCE2016-46 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | We have been developing a highly sensitive liquid-phase detection of biological targets using magnetic markers and HTS SQUID. In order to realize high sensitivity of this technique, it is necessary to decrease signal from free markers, which we call blank signal, as well as to increase signal from bound markers. Previously, we showed that dispersion process after magnetization is effective to decrease blank signal. In this study, we optimized reaction conditions when the dispersion process was introduced, i.e., density of magnetic markers and time used for reaction. We also measured diameter of magnetic markers before and after the magnetization so as to study the aggregation caused by the magnetic field. It was found that magnetic field accelerated aggregation of magnetic markers. Then, we calculated blank signal from the agglomerate of free markers by taking into account their Brownian relaxation. The calculated blank signal reasonably agreed with the experimental result. Finally, we measured the Brownian relaxation time of the bound markers, and found that the measured time was shorter than the theoretical expectation. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | Biosensor / Brownian Relaxation / Immunoassay / Magnetic Marker / HTS SQUID / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 116, no. 410, SCE2016-46, pp. 43-48, Jan. 2017. | |
| Paper # | SCE2016-46 | |
| Date of Issue | 2017-01-12 (SCE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | SCE2016-46 Link to ES Tech. Rep. Archives: SCE2016-46 |
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| Conference Information | |
| Committee | SCE |
| Conference Date | 2017-01-19 - 2017-01-19 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | SCE |
| Conference Code | 2017-01-SCE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Effect of reaction conditions on magnetic signal from magnetic markers in liquid-phase immunoassays |
| Sub Title (in English) | |
| Keyword(1) | Biosensor |
| Keyword(2) | Brownian Relaxation |
| Keyword(3) | Immunoassay |
| Keyword(4) | Magnetic Marker |
| Keyword(5) | HTS SQUID |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Kota Nakamura |
| 1st Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 2nd Author's Name | Yoshitsugu Takafuji |
| 2nd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 3rd Author's Name | Kota Irie |
| 3rd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 4th Author's Name | Teruyoshi Sasayama |
| 4th Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 5th Author's Name | Takashi Yoshida |
| 5th Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 6th Author's Name | Keiji Enpuku |
| 6th Author's Affiliation | Kyushu University (Kyushu Univ.) |
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| Speaker | Author-1 |
| Date Time | 2017-01-19 14:50:00 |
| Presentation Time | 25 minutes |
| Registration for | SCE |
| Paper # | SCE2016-46 |
| Volume (vol) | vol.116 |
| Number (no) | no.410 |
| Page | pp.43-48 |
| #Pages | 6 |
| Date of Issue | 2017-01-12 (SCE) |
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