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Paper Abstract and Keywords
Presentation 2017-04-21 11:35
Effect of Protein Adsorption Layers on Preparation of Hydroxyapatite-Coated Polystyrene Substrates Based on Biomimetic Process
Kazutoshi Iijima, Ayako Iizuka, Ryo Suzuki (Tokyo Univ. of Sci.), Hitomi Ueno-Yokohata, Nobutaka Kiyokawa (NCCHD), Mineo Hashizume (Tokyo Univ. of Sci.) SDM2017-7 OME2017-7
Abstract (in Japanese) (See Japanese page) 
(in English) We have developed a method to functionalize polystyrene (PS) substrate surfaces with hydroxyapatite (HAp) via protein adsorption layers such as human serum albumin (HSA) in simulated body fluid (SBF). In this study, the effect of protein adsorption layers on HAp deposition on PS substrate surfaces in SBF was evaluated using HSA, type I collagen, hen egg white lysozyme, and poly l-glutamic acid, an acidic protein analogue. Pretreatments with alternate soaking process (ASP) using solutions containing calcium ions and phosphate ions followed by incubation with SBF for 24 h resulted in HAp deposition on PS plates with protein adsorption layers, and the deposition behaviors were correlated with adsorption ability and charge state of proteins. Adhesion, proliferation, and differentiation of mesenchymal stem cells on HAp-coated PS plates were also investigated.
Keyword (in Japanese) (See Japanese page) 
(in English) Hydroxyapatite / Simulated Body Fluid / Polystyrene / Protein / Mesenchymal Stem Cell / / /  
Reference Info. IEICE Tech. Rep., vol. 117, no. 8, OME2017-7, pp. 31-34, April 2017.
Paper # OME2017-7 
Date of Issue 2017-04-13 (SDM, OME) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF SDM2017-7 OME2017-7

Conference Information
Committee OME SDM  
Conference Date 2017-04-20 - 2017-04-21 
Place (in Japanese) (See Japanese page) 
Place (in English) Tatsugochou Shougaigakushuu Center 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Organic molecular devices, silicon device, biotechnology, thin film device, novel material, evaluation method, etc 
Paper Information
Registration To OME 
Conference Code 2017-04-OME-SDM 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Effect of Protein Adsorption Layers on Preparation of Hydroxyapatite-Coated Polystyrene Substrates Based on Biomimetic Process 
Sub Title (in English)  
Keyword(1) Hydroxyapatite  
Keyword(2) Simulated Body Fluid  
Keyword(3) Polystyrene  
Keyword(4) Protein  
Keyword(5) Mesenchymal Stem Cell  
Keyword(6)  
Keyword(7)  
Keyword(8)  
1st Author's Name Kazutoshi Iijima  
1st Author's Affiliation Tokyo University of Science (Tokyo Univ. of Sci.)
2nd Author's Name Ayako Iizuka  
2nd Author's Affiliation Tokyo University of Science (Tokyo Univ. of Sci.)
3rd Author's Name Ryo Suzuki  
3rd Author's Affiliation Tokyo University of Science (Tokyo Univ. of Sci.)
4th Author's Name Hitomi Ueno-Yokohata  
4th Author's Affiliation National Research Institute for Child Health and Development (NCCHD)
5th Author's Name Nobutaka Kiyokawa  
5th Author's Affiliation National Research Institute for Child Health and Development (NCCHD)
6th Author's Name Mineo Hashizume  
6th Author's Affiliation Tokyo University of Science (Tokyo Univ. of Sci.)
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Speaker Author-1 
Date Time 2017-04-21 11:35:00 
Presentation Time 25 minutes 
Registration for OME 
Paper # SDM2017-7, OME2017-7 
Volume (vol) vol.117 
Number (no) no.7(SDM), no.8(OME) 
Page pp.31-34 
#Pages 4 
Date of Issue 2017-04-13 (SDM, OME) 

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