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Paper Abstract and Keywords
Presentation 2017-06-27 15:00
Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design
Tomoo Sato (PARI), Hiromasa Yamashita (Nihon Univ.), Yoshitaka Miyamoto (Nagoya Univ.), Kazuo Kurokawa (RIKEN), Toshio Chiba (Nihon Univ.) US2017-24
Abstract (in Japanese) (See Japanese page) 
(in English) In order to investigate the gene incorporation into cell by sonoporation using the optical microscope, the irradiation conditions of ultrsound were considered. The cultured cells (HepG2) with plasmid DNA (pEGFP-N3) and microbubbles (sonazoid), were exposure to the ultrasound. And the fluorescence intensity from Green Fluorescent Protein which was developed was measured. We assigned the following four factors to L9-orthogonal table of an experimental design and analyzed the factors which induce large the changes of the fluorescence intensity. [ (1) distance between ultrasound transmitter and bottom of microplate where the cells are cultured (2) intensity of exposing ultrasound, (3) exposing period, (4) duty ratio of exposing ultrasound ] The intensity of fluorescence of the GFP was shown to be influenced by the distance and the intensity of ultrasound.
Keyword (in Japanese) (See Japanese page) 
(in English) Fetal treatment, / Transgenesis / Sonoporation / Ultrasound / Microbubble / Experimental design / /  
Reference Info. IEICE Tech. Rep., vol. 117, no. 111, US2017-24, pp. 19-23, June 2017.
Paper # US2017-24 
Date of Issue 2017-06-20 (US) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
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All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee US  
Conference Date 2017-06-27 - 2017-06-27 
Place (in Japanese) (See Japanese page) 
Place (in English)  
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Paper Information
Registration To US 
Conference Code 2017-06-US 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design 
Sub Title (in English)  
Keyword(1) Fetal treatment,  
Keyword(2) Transgenesis  
Keyword(3) Sonoporation  
Keyword(4) Ultrasound  
Keyword(5) Microbubble  
Keyword(6) Experimental design  
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Keyword(8)  
1st Author's Name Tomoo Sato  
1st Author's Affiliation Port and Airport Research Institute (PARI)
2nd Author's Name Hiromasa Yamashita  
2nd Author's Affiliation Nihon University (Nihon Univ.)
3rd Author's Name Yoshitaka Miyamoto  
3rd Author's Affiliation Nagoya University (Nagoya Univ.)
4th Author's Name Kazuo Kurokawa  
4th Author's Affiliation Institute of Physical and Chemical Research (RIKEN)
5th Author's Name Toshio Chiba  
5th Author's Affiliation Nihon University (Nihon Univ.)
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Speaker Author-1 
Date Time 2017-06-27 15:00:00 
Presentation Time 25 minutes 
Registration for US 
Paper # US2017-24 
Volume (vol) vol.117 
Number (no) no.111 
Page pp.19-23 
#Pages
Date of Issue 2017-06-20 (US) 


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