Paper Abstract and Keywords |
Presentation |
2017-06-27 15:00
Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design Tomoo Sato (PARI), Hiromasa Yamashita (Nihon Univ.), Yoshitaka Miyamoto (Nagoya Univ.), Kazuo Kurokawa (RIKEN), Toshio Chiba (Nihon Univ.) US2017-24 |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
In order to investigate the gene incorporation into cell by sonoporation using the optical microscope, the irradiation conditions of ultrsound were considered. The cultured cells (HepG2) with plasmid DNA (pEGFP-N3) and microbubbles (sonazoid), were exposure to the ultrasound. And the fluorescence intensity from Green Fluorescent Protein which was developed was measured. We assigned the following four factors to L9-orthogonal table of an experimental design and analyzed the factors which induce large the changes of the fluorescence intensity. [ (1) distance between ultrasound transmitter and bottom of microplate where the cells are cultured (2) intensity of exposing ultrasound, (3) exposing period, (4) duty ratio of exposing ultrasound ] The intensity of fluorescence of the GFP was shown to be influenced by the distance and the intensity of ultrasound. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
Fetal treatment, / Transgenesis / Sonoporation / Ultrasound / Microbubble / Experimental design / / |
Reference Info. |
IEICE Tech. Rep., vol. 117, no. 111, US2017-24, pp. 19-23, June 2017. |
Paper # |
US2017-24 |
Date of Issue |
2017-06-20 (US) |
ISSN |
Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 |
Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) |
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US2017-24 |
Conference Information |
Committee |
US |
Conference Date |
2017-06-27 - 2017-06-27 |
Place (in Japanese) |
(See Japanese page) |
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Topics (in Japanese) |
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Paper Information |
Registration To |
US |
Conference Code |
2017-06-US |
Language |
Japanese |
Title (in Japanese) |
(See Japanese page) |
Sub Title (in Japanese) |
(See Japanese page) |
Title (in English) |
Investigation of irradiation condition for real-time monitoring of sonoporation, using experimental design |
Sub Title (in English) |
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Keyword(1) |
Fetal treatment, |
Keyword(2) |
Transgenesis |
Keyword(3) |
Sonoporation |
Keyword(4) |
Ultrasound |
Keyword(5) |
Microbubble |
Keyword(6) |
Experimental design |
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1st Author's Name |
Tomoo Sato |
1st Author's Affiliation |
Port and Airport Research Institute (PARI) |
2nd Author's Name |
Hiromasa Yamashita |
2nd Author's Affiliation |
Nihon University (Nihon Univ.) |
3rd Author's Name |
Yoshitaka Miyamoto |
3rd Author's Affiliation |
Nagoya University (Nagoya Univ.) |
4th Author's Name |
Kazuo Kurokawa |
4th Author's Affiliation |
Institute of Physical and Chemical Research (RIKEN) |
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Toshio Chiba |
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Nihon University (Nihon Univ.) |
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Speaker |
Author-1 |
Date Time |
2017-06-27 15:00:00 |
Presentation Time |
25 minutes |
Registration for |
US |
Paper # |
US2017-24 |
Volume (vol) |
vol.117 |
Number (no) |
no.111 |
Page |
pp.19-23 |
#Pages |
5 |
Date of Issue |
2017-06-20 (US) |
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