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| Presentation | 2021-12-22 15:50 [Poster Presentation] Thin sliced sample preparation and measurement protocol that enables the stable biological speed of sound evaluation Suguru Seto, Mai Ino, Kazuma Noguchi (Chiba Univ.), Kazuki Tamura (Hamamatsu Univ.), Shinnosuke Hirata, Kenji Yoshida, Tadashi Yamaguchi (Chiba Univ.)  US2021-57 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | Scanning acoustic microscopy (SAM) system allows acoustic characterization at the cell size level. There are two standard methods of preparing thin sliced samples, paraffin-embedded and frozen, and we have confirmed that the time of formalin fixation, which is often used in both methods, affects the speed of sound (SoS). In addition, the paraffin-embedded thin sliced samples have the problem of melting fat in the tissues. On the other hand, the freezing method is the absence of heat treatment, so it might be possible to evaluate the SoS of fat. This paper investigated the effect of the sample preparation on evaluating SoS in the freezing method. In particular, we focused on the half Karnovsky fixation method and examined the effect of the different fixation methods on the SoS. It was confirmed that half Karnovsky-fixation reduced tissue damage to the same extent as formalin fixation, and the absence of heat treatment improved the validity of SoS evaluation. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | quantitative ultrasound / acoustic microscopy / tissue characterization / speed of sound / liver / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 121, no. 310, US2021-57, pp. 51-55, Dec. 2021. | |
| Paper # | US2021-57 | |
| Date of Issue | 2021-12-15 (US) | |
| ISSN | Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | US2021-57 |
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| Conference Information | |
| Committee | EA US |
| Conference Date | 2021-12-22 - 2021-12-23 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Sojo University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | [Joint Meeting on Acoustics and Ultrasonics Subsociety] Engineering/Electro Acoustics, Ultrasonics, etc. |
| Paper Information | |
| Registration To | US |
| Conference Code | 2021-12-EA-US |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Thin sliced sample preparation and measurement protocol that enables the stable biological speed of sound evaluation |
| Sub Title (in English) | |
| Keyword(1) | quantitative ultrasound |
| Keyword(2) | acoustic microscopy |
| Keyword(3) | tissue characterization |
| Keyword(4) | speed of sound |
| Keyword(5) | liver |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Suguru Seto |
| 1st Author's Affiliation | Chiba University (Chiba Univ.) |
| 2nd Author's Name | Mai Ino |
| 2nd Author's Affiliation | Chiba University (Chiba Univ.) |
| 3rd Author's Name | Kazuma Noguchi |
| 3rd Author's Affiliation | Chiba University (Chiba Univ.) |
| 4th Author's Name | Kazuki Tamura |
| 4th Author's Affiliation | Hamamatsu Univ. School of Medicine (Hamamatsu Univ.) |
| 5th Author's Name | Shinnosuke Hirata |
| 5th Author's Affiliation | Chiba University (Chiba Univ.) |
| 6th Author's Name | Kenji Yoshida |
| 6th Author's Affiliation | Chiba University (Chiba Univ.) |
| 7th Author's Name | Tadashi Yamaguchi |
| 7th Author's Affiliation | Chiba University (Chiba Univ.) |
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| Speaker | Author-1 |
| Date Time | 2021-12-22 15:50:00 |
| Presentation Time | 120 minutes |
| Registration for | US |
| Paper # | US2021-57 |
| Volume (vol) | vol.121 |
| Number (no) | no.310 |
| Page | pp.51-55 |
| #Pages | 5 |
| Date of Issue | 2021-12-15 (US) |
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