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Paper Abstract and Keywords
Presentation 2022-02-10 14:25
Low-cost fabrication of structured illumination microscope using 3D-printed flexure stage
Daigo Fujiwara, Tatsunosuke Matui (Mie Univ.) OME2021-57 Link to ES Tech. Rep. Archives: OME2021-57
Abstract (in Japanese) (See Japanese page) 
(in English) The OpenFlexure stage and microscope, which is open source and can be replicated by 3D printing, uses a flexure mechanism to achieve extremely high positioning control with submicron precision. However, it is an ordinary optical microscope and does not have depth resolution. In this study, we worked on the improvement of the microscope to a structured illumination microscope with optical sectioning capability by adding printed parts for structured illumination. The additional parts were designed using an open CAD software, OpenSCAD, and were fabricated by 3D printing. The stage motion control, image acquisition, and analysis can be performed on a Raspberry Pi using Python. The Raspberry Pi can be remotely controlled from a PC via Wi-Fi. Optical sectioning images of fibers in a nonwoven mask were obtained using a structured illumination microscope.
Keyword (in Japanese) (See Japanese page) 
(in English) 3D print / flexure stage / structured illumination microscope / SIM / optical sectioning / depth resolution / /  
Reference Info. IEICE Tech. Rep., vol. 121, no. 367, OME2021-57, pp. 14-19, Feb. 2022.
Paper # OME2021-57 
Date of Issue 2022-02-03 (OME) 
ISSN Online edition: ISSN 2432-6380
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
Download PDF OME2021-57 Link to ES Tech. Rep. Archives: OME2021-57

Conference Information
Conference Date 2022-02-10 - 2022-02-10 
Place (in Japanese) (See Japanese page) 
Place (in English) Online 
Topics (in Japanese) (See Japanese page) 
Topics (in English) Organic Molecular Electronics 
Paper Information
Registration To OME 
Conference Code 2022-02-OME-DEI 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Low-cost fabrication of structured illumination microscope using 3D-printed flexure stage 
Sub Title (in English)  
Keyword(1) 3D print  
Keyword(2) flexure stage  
Keyword(3) structured illumination microscope  
Keyword(4) SIM  
Keyword(5) optical sectioning  
Keyword(6) depth resolution  
1st Author's Name Daigo Fujiwara  
1st Author's Affiliation Mie University (Mie Univ.)
2nd Author's Name Tatsunosuke Matui  
2nd Author's Affiliation Mie University (Mie Univ.)
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Speaker Author-1 
Date Time 2022-02-10 14:25:00 
Presentation Time 15 minutes 
Registration for OME 
Paper # OME2021-57 
Volume (vol) vol.121 
Number (no) no.367 
Page pp.14-19 
Date of Issue 2022-02-03 (OME) 

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