| Paper Abstract and Keywords |
| Presentation |
2013-04-26 09:50
Orientation of a bacteriorhodopsin thin film deposited by dip coating technique
-- Chirality in SHG and absolute orientation -- Toshiki Yamada, Yoshihiro Haruyama, Katsuyuki Kasai, Toshifumi Terui, Shukichi Tanaka, Takahiro Kaji (NICT), Hiroshi Kikuchi (NHK), Akira Otomo (NICT) SDM2013-3 OME2013-3 |
| Abstract |
(in Japanese) |
(See Japanese page) |
| (in English) |
Bacteriorhodopsin (bR) is a protein containing a covalently bound protonated Schiff base retinal chromophore, found in the purple membrane of Halobacterium salinarium. It is well-known that the bR exhibits a light-driven proton pump function, and photo-sensor devices having the bR thin film show a transient photo-current signal under the “on” or “off” of photo-irradiation. It is important to understand the orientation of bRs in the bR thin film for evaluating such devices and elucidating the transient photo-current signal characteristics. In this paper, we show that by observing SHG interference the bR thin films prepared by a simple dip coating technique have a polar orientation with chiral C symmetry. We found some characteristic phenomena in the chiral SHG by observing the interference by using two coupled chiral SH active films. Furthermore, we performed SHG interference measurements to clarify the absolute orientation of bRs in the bR thin film, and found that bRs tend to have a preference to orient with the cytoplasmic side away from the substrate and extracellular side toward the substrate. |
| Keyword |
(in Japanese) |
(See Japanese page) |
| (in English) |
Bacteriorhodopsin / SHG interference / dip-coating technique / chiral SHG / Absolute orientation / / / |
| Reference Info. |
IEICE Tech. Rep., vol. 113, no. 18, OME2013-3, pp. 9-13, April 2013. |
| Paper # |
OME2013-3 |
| Date of Issue |
2013-04-18 (SDM, OME) |
| ISSN |
Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 |
Copyright and reproduction |
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SDM2013-3 OME2013-3 |