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Paper Abstract and Keywords
Presentation 2022-07-08 14:40
[Short Paper] Automatic Cell Tracking in 4D Arabidopsis Root Microscopic Images Using Genetic Algorithm
Yu Song, Yutaro Iwamoto (Ritsumeikan Univ.), Tatsuaki Goh (NAIST), Yohei Kondo (ExCELLS), Keiji Nakajima (NAIST), Yen-wei Chen (Ritsumeikan Univ.) MI2022-34
Abstract (in Japanese) (See Japanese page) 
(in English) Single-particle tracking (SPT) is a crucial progress to discover unknown principles of plants. Through accurate tracking of each particle on time-lapse images of biological samples taken by microscopes, many hypotheses in biological field can thus be verified. A commonly used method for tracking is called linear assignment problem (LAP) tracker. LAP tracker is well suited for particles undergoing Brownian motion. However, in our task, we are going to track the cortex cells of Arabidopsis root on 3D time-lapse (4D) images, where cells of Arabidopsis root are densely arranged. This makes LAP tracker ineffective when considering the distances costs of links. To realize accurate SPT on this task, we propose an automatic tracking method using genetic algorithm (GA). Based on the shape information we know about Arabidopsis roots, a 3D image at a frame can be viewed as 8 lines of cells. Therefore, we turn the SPT into the tracking on each of 8 lines in the time axis. Due to the elongation of cells, each line is not a straight line but more like a curve. For each frame, we use a GA to select an optimal plane to project cells. The projected cells are well separated to 8 regions, where each region represents a line of cells. After clustering the projected cells into 8 lines, we do the tracking based on 8 lines of cells. Finally, we operate SPT of each cortex cell. Through experiments, the proposed method realizes stable and accurate tracking of Arabidopsis root cortex cells.
Keyword (in Japanese) (See Japanese page) 
(in English) Arabidopsis Root / Cell Tracking / Genetic Algorithm / Single-Particle Tracking / / / /  
Reference Info. IEICE Tech. Rep., vol. 122, no. 98, MI2022-34, pp. 10-15, July 2022.
Paper # MI2022-34 
Date of Issue 2022-07-01 (MI) 
ISSN Online edition: ISSN 2432-6380
Copyright
and
reproduction		 All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MI  
Conference Date 2022-07-08 - 2022-07-09 
Place (in Japanese) (See Japanese page) 
Place (in English)  
Topics (in Japanese) (See Japanese page) 
Topics (in English) Medical imaging, recoginition, etc. 
Paper Information
Registration To MI 
Conference Code 2022-07-MI 
Language English 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Automatic Cell Tracking in 4D Arabidopsis Root Microscopic Images Using Genetic Algorithm 
Sub Title (in English)  
Keyword(1) Arabidopsis Root  
Keyword(2) Cell Tracking  
Keyword(3) Genetic Algorithm  
Keyword(4) Single-Particle Tracking  
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1st Author's Name Yu Song  
1st Author's Affiliation Ritsumeikan University (Ritsumeikan Univ.)
2nd Author's Name Yutaro Iwamoto  
2nd Author's Affiliation Ritsumeikan University (Ritsumeikan Univ.)
3rd Author's Name Tatsuaki Goh  
3rd Author's Affiliation Nara Institute of Science and Technology (NAIST)
4th Author's Name Yohei Kondo  
4th Author's Affiliation Exploratory Research Center on Life and Living Systems (ExCELLS)
5th Author's Name Keiji Nakajima  
5th Author's Affiliation Nara Institute of Science and Technology (NAIST)
6th Author's Name Yen-wei Chen  
6th Author's Affiliation Ritsumeikan University (Ritsumeikan Univ.)
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Speaker Author-1 
Date Time 2022-07-08 14:40:00 
Presentation Time 20 minutes 
Registration for MI 
Paper # MI2022-34 
Volume (vol) vol.122 
Number (no) no.98 
Page pp.10-15 
#Pages 6 
Date of Issue 2022-07-01 (MI) 

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