Paper Abstract and Keywords |
Presentation |
2023-02-28 15:00
Temperature dependence of specific capacitance extracted from repeated EIS measurements of free-standing DPPC bilayer membranes Shoya Ueda, Yoshihiro Shimada, Daiki Hiramatsu, Masataka Moriya (UEC Tokyo), Daisuke Tadaki, Ayumi Hirano-Iwata (Tohoku Univ.), Yoshinao Mizugaki (UEC Tokyo) CPM2022-102 |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
Bilayer lipid membranes (BLMs) formed in micropores are referred to as free-standing BLMs. The folding method is a technique to form a free-standing BLM. Recently, the yield of free-standing BLM formation has been improved by using micropores fabricated in Si/SiNx chips, whereas BLM capacitance should be extracted by means of electrochemical impedance spectroscopy (EIS) with equivalent circuit analysis. Among a lot of phospholipids, DPPC is known to undergo phase transitions at around room temperature, resulting in discontinuous changes of the BLM capacitance. In this study, EIS measurements were performed repeatedly for free-standing DPPC bilayers while changing the temperature. The temperature dependence of the area-specific capacitance was extracted from equivalent circuit analysis of the EIS results. As a result, although no clear discontinuous capacitance changes were observed, inflection points were confirmed around the phase transition temperatures. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
lipid bilayer membrane / folding method / equivalent circuit analysis / phase transition / / / / |
Reference Info. |
IEICE Tech. Rep., vol. 122, no. 392, CPM2022-102, pp. 62-65, Feb. 2023. |
Paper # |
CPM2022-102 |
Date of Issue |
2023-02-21 (CPM) |
ISSN |
Online edition: ISSN 2432-6380 |
Copyright and reproduction |
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CPM2022-102 |
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