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| Presentation | 2023-02-28 13:00 Construction of an evaluation platform for specific membrane capacitance through the combination of optical microscopy and artificial cell membrane system Hironori Kageyama, Maki Komiya, Teng Ma, Ayumi Hirano-Iwata (Tohoku Univ.)  CPM2022-95 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | Ion channels, membrane-embedded proteins, are responsible for selective ion permeation through the cell membrane. Recently, it has been reported that for some channels, their membrane permeability is affected by the thickness of the cell membrane. Therefore, it has been desirable to establish a membrane thickness evaluation system using artificial cell membranes that are more controllable than actual cell membranes to investigate the correlation between the thickness of cell membranes and ion channel function. The membrane thickness is generally evaluated in terms of specific membrane capacitance, and the simultaneous recording of membrane area and capacitance is necessary to calculate the value. However, the Folding method, which requires less organic solvent and can measure under conditions closer to biological membranes, has been difficult to integrate with existing upright and inverted microscopes due to problems such as increased current noise. In this study, we developed a new measurement system that combines artificial cell membranes formed by the Folding method with microscopic observation to achieve high-precision analysis of specific membrane capacitance, which is an index for evaluating membrane thickness. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | artificial cell membrane / lipid bilayer / folding method / specific membrane capacitance / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 122, no. 392, CPM2022-95, pp. 39-40, Feb. 2023. | |
| Paper # | CPM2022-95 | |
| Date of Issue | 2023-02-21 (CPM) | |
| ISSN | Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | CPM2022-95 |
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| Conference Information | |
| Committee | CPM |
| Conference Date | 2023-02-28 - 2023-02-28 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tokyo University of Technology |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | CPM |
| Conference Code | 2023-02-CPM |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Construction of an evaluation platform for specific membrane capacitance through the combination of optical microscopy and artificial cell membrane system |
| Sub Title (in English) | |
| Keyword(1) | artificial cell membrane |
| Keyword(2) | lipid bilayer |
| Keyword(3) | folding method |
| Keyword(4) | specific membrane capacitance |
| Keyword(5) | |
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| 1st Author's Name | Hironori Kageyama |
| 1st Author's Affiliation | Tohoku University (Tohoku Univ.) |
| 2nd Author's Name | Maki Komiya |
| 2nd Author's Affiliation | Tohoku University (Tohoku Univ.) |
| 3rd Author's Name | Teng Ma |
| 3rd Author's Affiliation | Tohoku University (Tohoku Univ.) |
| 4th Author's Name | Ayumi Hirano-Iwata |
| 4th Author's Affiliation | Tohoku University (Tohoku Univ.) |
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| Speaker | Author-1 |
| Date Time | 2023-02-28 13:00:00 |
| Presentation Time | 15 minutes |
| Registration for | CPM |
| Paper # | CPM2022-95 |
| Volume (vol) | vol.122 |
| Number (no) | no.392 |
| Page | pp.39-40 |
| #Pages | 2 |
| Date of Issue | 2023-02-21 (CPM) |
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